Study of Serum Endothelin - 1 Variation in Sickle Cell Disease

BACKGROUND: Hb level decreased and Serum Endothelin-1 (ET-) level have been observed increased in sickle cell disease. The biological mechanism of synthesis and regulation of the Endothelin-1 (ET-) will be clear. The aim of the research study is to provide an overview of the Hb, CBC count, and Sr. Endothelin-1 level changes and its significance in sickle cell disease. METHODS: Hematological values Complete blood count (CBC) was measured on fully Automatic blood cell counter hematology analyzer (Coulter LH 780), and HmX Hematology Analyzer with Autoloader. But in this study, we have concentrated on the variables like, Haemoglobin - (Hb), WBC, RBC, PCV and MCV count. All the values were compared to the reference values. The ET-1 was determined by using enzyme-immunoassay (ELISA kit) method, specifically designed for direct measurement of plasma ET-1. The sensitivity of the assay is 0.5pg/ml, and the cross-sensitivity of the antibodies used is reported to be less than 1 % with big ET-1. RESULTS: The study showed that, the significant variations in CBC parameters were observed. The White Blood Cells counts (WBC), and Sr. ET-1levels were significantly increased. But the other indices like, Red Blood Cells (RBCs), PCV, and MCV were found significantly decreased with the controls in SCD. P 0.05was considered to be statistically significant. CONCLUSION: The study concluded that, there is an extremely significant difference between mean Serum Hb, CBC and Endothelin-1 (ET-) levels in cases and healthy controls. So the Hb, CBC count and Serum Endothelin-1 level can be used as a clinical bio-marker for the diagnosis of Sickle Cell Disease.