Antioxidant, In-Vitro Anti-Inflammatory and Anti-Alzheimer Activities of Methanolic Fraction of Salvia Miltiorrhiza Flower

The present study aimed to investigate the antioxidant, in-vitro anti-inflammatory and Anti-Alzheimer activities of methanolic extract of Salvia miltiorrhiza (MESM) in various in vitro models. Preliminary phytochemical screening was also performed. Antioxidant activity and free radical scavenging activity of MESM has been evaluated by employing various in vitro models including reducing power, Total antioxidant activity, DPPH radical, ABTS radical, superoxide radical, nitricoxide, hydrogen peroxide, hydroxyl radical scavenging activity and metal chelating activity. Anti-inflammatory activity has been evaluated by various in vitro methods including protein denaturation, anti-proteinase action and membrane stabilization. The plant extract (MESM) revealed significant antioxidant activity with lower IC50 values. Metal chelating activity was found poor. In inflammation modelS, MESM demonstrated significant anti-inflammatory activity. Acetylcholinesterase (AChE) inhibitory activity was also estimated. Anti-Alzheimer profile was also evaluated along with the necessary observations using neuroblastoma cell lines. Results demonstrated a neuroprotective profile of the methanol extract of Salvia miltiorrhiza as well as revealed a protective efficacy in Alzheimers disease (AD). The results indicated that the plant could be a potential source of antioxidant and could find a use in the herbal therapy of inflammation also, which further supports the protective efficacy in AD