Study and Effect of Different Tissue Culture Media on the Propagation of Rice by Anther Culture

The investigation was conducted to standardize culture media for callus induction and regeneration from anther derived callus of Sona Masuri (Oryza sativa subspindica), NLR-145 (Oryza sativa subspindica, Pusa-834 (IET-11674) rice varieties. Before inoculation of the anthers, a cold pre-treatment of 50C for 8 days was given to the selected panicles. Anther culture is useful technique in rice breeding programms. as production of haploid plants speeds up the breeding cycle by fixing homozygosis in one generation. Rice is the worlds single most important food crop and is the primary food for more than one third of the worlds population. Production and consumption are concentrated in Asia where more than 90 % of all rice is produced and consumed. MS basal medium supplement with 30 and 40 g/l sucrose, 8 g/l agar and different plant growth regulator were used. Rice was cultured in different ratio of BAP: IAA (10: 10, 10: 5, 5: 10, and 5: 5 M) in combination. Development was not identical in all the treatments, cultured tissues were observed in treatments about a week after culture. Results showed that the treatments containing 10 M BAP in combination with 10 M IAA is suitable for callus induction. While the tissue formed in 5: 5 M BAP: IAA was found to be delicate, short. Also, the tissue formed in 5: 10 BAP: IAA was delicate, short but was higher in density as compared to treatment contained 5: 5 M BAP: IAA