Ethanol Extract of Black Rice (Oryza sativa L) Bran Reduced Expression of RAGE in Skin of Wistar Rat Exposed with UVA and UVB

Aging is ongoing process and aging in skin is a most common problem which still undergoes research for aging prevention. In ageing there are 2 theories, first wear and tear theory and second programming theory. The first is including DNA damage, glycation, and free radical. Exposure of UV increase expression of AGE in skin followed by increasing of its receptor (RAGE) in fibroblast and followed by activation of NF-kB and increasing MMP and eventually collagen degradation which is hallmark of skin aging. Skin aging can be prevented by anti-oxidant like anthocyanin and black rice bran rich of anthocyanin with anti-inflammation and anti-glycation activity. Based on our review there is no data about effect of black rice bran from Jogjakarta with animal model of skin aging. Thirty-six Wistar rats (Rattus novergicus) with 8-12 weeks old and 200-250 gr weight were randomly chosen and adapted for 7 days. Rats were divided into 2 groups and exposed with 20 mJ/cm2 UVA and UVB for 4 weeks, five times a week. Control group only exposed to UVA and UVB and Treatment Group exposed to UVA and UVB with orally 300 mg/kg weight extract. After 4 weeks the rats were sacrificed and RAGE expression in skin was determined by immunohistochemistry. Independent T-test analysis found significant difference in percentage of fibroblast that expressed RAGE (p