Crown Dentin Analysis using Fourier-Transform Infrared Spectroscopy with Attenuated Total Reflectance (FTIR-ATR)

Dentin determins the mechanical properties of teeth and distributes the mechanical pressure. The aim of our study was to determine the changes that take place in samples of crown dentin in vital and devitalized teeth using FTIR-ATR. The samples used were taken from young crown dentin, old crown dentin and crown dentin from endodontically treated teeth (ETT). The spectroscopic images obtained showed typical spectres for dentin, with absorbance peaks between 800 3500/cm-1. No differences in the dentin composition were observed, regardless of age and endodontic treatment. No presence of new chemical bonds was noted and all of the basic dentin components were detected. The conducted analysis showed that crown dentin consists of hydroxyapatite with good crystallinity, a high concentration of phosphate groups and undamaged organic matrix.