Effect of Mutagens on Germination Percentage, Seeding Height and Seedling Injury in M1 Generation of Vicia fabaL

Vicia faba L. is a flowering plant belonging to family Fabaceae which reflects all properties of a legume crop such as soil improvement and benefit of human health. It has an outstanding production scale in approximately 58 countries and rating third in engenderment. Faba beans magnification is facilated in any climatic and soil condition. It functions as a precursor of dopamine a neurotransmitter which can be used to cure Parkinson�s disease and also participates in biological nitrogen fixation thereby incrementing soil fertility. Vicia faba seeds contains high nutritional value and opulent in protein, carbohydrates, in volute vitamins, folic acid, niacin and vitamin C, dietary fibres and macro and micro nutrients. The present research work was conducted to study morphological changes and chlorophyll mutation in leaves of Vicia faba by single and combined treatment of Gamma rays and Ethyl Methanesulphonate. Screening of mutation was carried out in M1 generation of Vicia faba variety Wal Kokan Bhushan. The seeds were given individual doses/concentrations of Gamma rays (240 Gy, 300 Gy, 360 Gy, 420 Gy) and EMS (0.25 %, 0.50 %, 0.75 %, 1 %). Seeds were sown to raise M1 generation. Germination percentage and seedling height was increases with decreased in doses/concentrations of different mutagens. Highest Seedling injury was observed in higher doses of Gamma rays combined with lower doses of EMS. Different chlorophyll mutation observed were xantha, viridis, aurea, maculate. The frequency of chlorophyll mutation increased with decreases doses/concentrations of both single and combined treatments.