Detection of Inducible Clindamycin Resistance and Methicillin Resistance in Staphylococcus species from Various Clinical Samples

Staphylococcus species is an important cause of nosocomial and community acquired infections worldwide. Clindamycin is an alternative agents used to treat Staphylococcal infections. Accurate identification of clindamycin resistance is important to prevent therapeutic failure. Unfortunately, inducible CL resistance is not detected by standard susceptibility tests. The aim of the present study was to detect the prevalence of inducible clindamycin and methicillin resistance among clinical isolates of Staphylococcal species via antibiotic sensitivity test. Total 129 Staphylococcal isolates were tested for antimicrobial susceptibility testing by as per guidelines. For detection of MRSA cefoxitin disc and for inducible clindamycin resistance, D test was performed. Out of 129 samples, 101 were Staphylococcus aureus and 28 were Coagulase negative Staphylococcus (CoNS). Out of which 61.3 % were MRSA and 38.6 % were MSSA. Inducible clindamycin resistant was detected in 30.6 % and MS phenotype in 42.7 %. D-test should be routinely performed for every Staphylococcus isolates otherwise clindamycin resistance may misinterpreted as clindamycin sensitive resulting in therapeutic failure.