Effect of Dentin Deproteinization using Bromelain Enzyme and Sodium Salt of Vitamin C on Microleakage in Class V Composite Restorations: An in Vitro Study

Aim: The aim of the present study was to evaluate the effect of surface treatments using sodium hypochlorite and sodium salt of Vitamin C when compared to Bromelain on microleakage of class V composite restorations. Methods and Material: 60 intact premolars were collected and class V cavity preparations were done on buccal surfaces and were divided into three groups of 20 each based on surface treatments done as Group A: acid etching alone, Group B: acid etching followed by hypo-sodium salt of Vitamin C, Group C: acid etching followed by Bromelain. Interfacial analysis of 5 randomly selected samples from each group were observed under Scanning electron microscope (SEM). Remaining 15 samples of each group were prepared for dye penetration and microleakage was analysed using stereomicroscope. Statistical analysis: statistical analysis was performed with PostHoc test and Kruskal Wallis using SPSS version 20. Results: The samples that were treated with Bromelain showed significantly least microleakage when compared to those treated with a combination of sodium hypochlorite- sodium salt of Vitamin C and acid etching alone. Conclusions: Bromelain surface treatment showed least microleakage with no gaps between the dentin and resin surface on observation under SEM.