Preparation Thymolas Microemulsion and Oil/Water Emulsion and Evaluation its Pre-Transplanting Nematicidal Activity against Root-Knot Nematode Meloidogine incognita

Thymol (crystal) an essential oil prepared as microemulsion (ME10 %) and oil/water emulsion (EW10 %), the two formulas were passed successfully of all the physico-chemical properties testes, and the particle size analysis. Compared the nematicidal efficacy of both formulas as post-emergence and pre-transplanting application under greenhouse conditions was determined. The results of post- emergence trial showed severe phytotoxicity of both formulas on cucumber treated seedling, and the ME10 % showed more nematicidal efficacy on Meloidogyne incognita than EW10 %, it showed as reducing in galls number on cucumber seedling roots comparing with untreated seedling. Pre-transplanting trials results showed no phytotoxicity on cucumber seedling planted after 3 or 7 days of both formulas application. However both of them gave excellent protection for cucumber seedling at 1000 and 2000 ppm as indicating by inhibition percent of formed galls, fresh weight of roots and fresh weight of shoots. In all bioassay trials the microemulsion formulation was better in efficacy against nematode than oil in water emulsion that is due to its smaller particle size and other physical properties like thermodynamically stable.