Molecular Cloning of Flavone Synthase I Gene from Cumin (Cuminum Cyminum)

Cuminum cyminum. L is one of the member Apiaceae family. This plant has anticancer and antioxidant traits. The main purpose of this research is cloning of flavone synthase I gene from Caraway Cumin (Cuminum cyminum) indigenous in Iran. In order to cloning FNSI after extraction of total RNA, cDNA Library were constructed using oligo dT primers and gene amplification of FNSI was performed. After cutting the amplified gene cloned in expression plasmid PET28a and was transferred to the DH5 bacterial utilizing by heat shock method. Screening of transformed bacteria was done on medium containing kanamycin and at least to confirm the gene cloning method for determination of the cloned genes, gene amplified of the gene using specific primers and cutting plasmid by specific restriction enzymes and analyzed of the products on agarose gel was used. In this experiment, after all the cloning steps, the 6kb and 1 kb bands were observed on the electrophoreses gel plasmid and the flavone synthase I gene, respectively, confirming the cloning of this gene in pET28a plasmid. Given that flavones are important in plants as well as being of high pharmaceutical value, having a cloned set of flavone synthase genes can be of high pharmaceutical and commercial value.