Genotoxicity Screening of the Crude Ethanolic Extract of Artocarpus heterophyllus (langka) Unripe Fruit Using Allium cepa (Onion) Assay

The crude ethanolic extract of Artocarpus heterophyllus (langka) unripe fruit was found out to lower blood sugar in alloxan-induced hyperglycemic white mice (Mus musculus). This study was conducted to determine the genotoxicity (mitotic index (MI) and percent chromosomal aberration ( %CA) of the crude ethanolic extract of A. heterophyllus unripe fruit using A. cepa (onion) assay. Onions with 1.5-2.0cm length of the roots were grown in different concentrations of A. heterophyllus extract. The 3 roots with a length of 2mm from the tips were harvested and fixed. Squash preparations were made for each of the concentration, positive and negative control in 3 replicates and 3 trials. Results showed that 15mg (MI=0.782+0.022, %CA=0.06+0.003) and 20mg (MI= 0.754+0.017, %CA=0.04+0.019) of the crude ethanolic extract per ml of ml of distilled water did not show genotoxicity based on the mitotic index (MI) and %chromosomal aberration (CA). They were comparable to the negative control used (dist. water) (MI=0.756+0.028, CA=0.08+0.022) (P