In Vitro Propagation and Sulforaphane Content Analysis Brassica oleraceaevar. Botrytis with TreatmentKinetin and Methionine

Brassica oleraceae var. Botrytis is a vegetable plant that is quite popular in Indonesia which has high economic value because it contains sulforaphane which has benefits to prevent cancer. This research aims to obtain the best concentration of kinetin and methionine in in vitro culture Brassica oleraceae var. Botrytis, and obtain the best concentration of kinetin and methionine for enhancement of sulforaphane from in vitro culture Brassica oleraceae var. Botrytis. The results showed that the concentration to obtain the best amount of shoot and leaf number was in the treatment of 5 mg/L kinetin without methionine, while the concentration to obtain the best plant height on the treatment of 100 mg/L methionine without kinetin, and the best plant fresh weight was at treatment combination of 10 mg/L kinetin and 50 mg/L methionine. The plant fresh weight of Brassica oleraceae var. Botrytis is negatively correlated with sulforaphane content in it. The treatment of 50 mg/L of methionine without kinetin was the best treatment in producing a high sulforaphane content of 47.7 ng/g wet weight.