Study on In Vitro Pollen Germination on Jatropha integerrima Jacq. Pollen

Jatropha integerrima Jacq. is known for its ornamental flower since long. But less is known about its pollen viability and in vitro pollen germination. The objective of this study was to assess the pollen viability and in vitro pollen germination in Jatropha integerrima Jacq. The pollens were collected from three flowering stages- pre anthesis, anthesis and post anthesis to evaluate the pollen viability, by TTC dye method. The flowering stage which showed maximum percentage pollen viability was taken into account for in vitro germination studies. Two culture mediums were used to assess in vitro pollen germination- Liquid medium (M1) and Semi solid medium (M2). In both the mediums maximum pollen germination was obtained in 20 % sucrose, 0.04 % Boric acid and 0.03 % Calcium chloride. But in comparison to M1, M2 medium gave more percentage pollen germination in the respective concentrations.