RP-HPLC Analysis of Flavonoids in Marsilea Minuta L

The present study was investigated for the presence of phenolic compounds in the plant extract of Marsilea minuta L. The studies on the phenolics or flavonoids by RP-HPLC analysis showed the presence of five flavonoid compounds such as gallic acid, caffeic acid, rutin, quercetin and ferulic acid. The chromatographic separations at retention time (Rt) for gallic acid (Rt - 5.675), Caffeic acid (Rt 9.475), Rutin (Rt -11.017), Quercetin (Rt -12.175) and Ferulic acid (Rt - 23.808). The individual flavonoid content for all the flavonoids were calculated from the corresponding calibration curve and the obtained values were compared with the standards. Hence these plant extracts with the property of phenolic compounds can be recommended for their use as an alternative anti-infective agent in natural medicine for the treatment of various diseases.