Evaluation of Topical Application of Propolis, Black Seeds and Honey on Oral Mucosal Healing in Rabbits (Histological and Immunohistochemical Study on TGF-?3)

Background Wound healing is classically characterized by the transient development of granulation tissue that supports rapid proliferation, migration, and differentiation of the adjacent epithelium. The transient reactive stroma includes vascularization of the wound, infiltration of inflammatory cells, and differentiation of the dermal fibroblasts. Growth factors released in the traumatized area stimulating the growth of epithelial cells and fibroblasts, initiate the formulation of new blood vessels, and Improved glucose level. The aim of the present study was to evaluate effect of mixture of propolis, black seed and honey on oral wound healing. Materials and methods Twelve New Zealand male rabbits were used in this study, they were divided into three groups according to 3, 7, and10 days healing intervals (4 animals for each group). All animals were subjected to alloxan injection to induced diabetes which was controlled by insulin. Application of a mixture of propolis, black seed and honey was done at wound of right side of check mucosa (Experimental), whereas left wound site (Control) was left to heal spontaneously. Histological and immunohistochemical study on TGF-3, assessment was performed for all groups. Results Histological and immunohisto chemical findings of this study showed that re-epithelialization, and remodeling of dermal fibrous connective tissue were accelerated after topical application of a mixture of propolis, black seed, and honey at wound site supported by the positive expression of TGF-3 by the cells at wound site. Conclusion Topical application of a mixture of propolis, black seed and honey was effective in wound healing of controlled diabetics.