Isolation, Screening and Production of Biosurfactant by PSEUDOMONAS AEROGINOSA SD4 Using Various Hydrocarbon Sources

Rising environmental concerns lead to emergence of biosurfactants as a potential alternative to the synthetic surfactants. In this study a total of 65 strains isolated from petrol bunk soil were screened using oil spreading method. Among the isolates four strains TD3, TD4, SD3 and SD4 showing higher Biosurfactant activity were selected, purified and subcultured on Psuedomonas selective agar plate. Of these four strains selected, isolate SD4 had maximum Biosurfactant activity. The isolate SD4 was identified as Pseudomonas aeroginosa strain by morphological, biochemical and with 16s rRNA analysis. Maximum Biosurfactant production was obtained at pH 7 and at incubation time of 72 hours. On analysis with diesel, petrol and kerosene at 2 % (v/v), P. aeroginosa SD4 exhibited maximum Biosurfactant production utilizing diesel incorporated into the production medium with highest E24 value (53-86 %) with olive oil, castor oil, coconut oil and sunflower oil respectively. Further the biosurfactant of 80 mg was extracted per ml of medium with diesel source and the Biosurfactant was characterized as rhamnolipid with phenol-sulphuric test and TLC analysis. Thus, this work emphasis the suitability of Pseudomonas aeruginosa SD4 further to be explored in the area of environmental and industrial application.