Production Partial Purification and Industrial Applications of Alkaline Protease Produced by Bacillus Lichenifomis

Proteases are very important industrial enzymes, which contribute maximum of the total world enzyme market. Besides their use in normal physiology, proteases are used in various industries including pharmaceuticals, detergents, food and waste processing. The most common and widely used bacteria belongs to the genus Bacillus. The aim of the work is based upon an extracellular alkaline protease produced by Bacillus licheniformis, under optimized conditions. The alkaline protease production was optimized. The optimum day 2 days, pH 8.5, temp 40c, were determined. The enzyme was further partially purified using acetone, followed by dialysis (75 kDa) and sephadox G100 column chromatography. The molecular weight of the enzyme was found to be 320KDa using SDS PAGE. The extracted enzyme was used for hair removal on goat skin. The extracted enzyme was found to be better agent to remove the hair efficient as an eco friendly method. This property of the enzyme is very useful for application in leather industry.