Applications of Immobilized Lipase enzyme produced from Pseudomonas aeruginosa

Lipases (Triacylglycerol acylhydrolases, EC 3.1.1.3) catalyze the enzymatic hydrolysis of triacylglycerol into diacylglycerol& free fatty acids. Due to the wide range of applications of Lipases in various industries like Detergent, Leather, Textiles, Food, Pharmaceuticals, etc. , Lipases serves as one of the most important enzymes in its class. The present study investigates the potentials of partially purified and immobilized extracellular Lipase enzyme produced from SP45 (Pseudomonas aeruginosa) isolated from oil contaminated soil samples for various applications. Immobilized SP45 Lipase was used for the enzymatic hydrolysis of edible oils and also for the detection of lipolysis of butter fats. Hydrolysis of edible oils reveals the fact that SP45 Lipase immobilized in sodium alginate beads exhibits maximum hydrolytic activity of 11.6667 U/ml/min against Groundnut oil when compared with other edible oils used in the study. As far as detection of lipolysis of butter fats was concerned, the copper soap test prominently showing bluish green zone around the colonies of SP45 (Pseudomonas aeruginosa) explains clear indication of strong lipolytic activity. In addition to that formation of clumps of butter fats were also observed which was caused due to the lipolysis reaction by immobilized SP45 Lipase enzyme. This clearly explains that immobilized SP45 Lipase enzyme exhibits the properties of lipolysis of butter fats.