Biosynthesis, Characterization, Antimicrobial Efficacy of AuNps and its Antifouling Application on Metal Coupons by Cajanuscajan(L.)Millsp.

Marine biofilms formed due to adhesion of bacteria and other microorganisms on submerged surfaces are generally considered to be a major form of microfouling. Subsequent attachment of larvae of higher organisms like barnacles, mussels, and so forth, on marine biofilms, causes macrofouling. Several approaches have been used to prevent micro- and macrofouling Hence, the present study is focused on the biosynthesis of AuNPs using Leaf extract of Cajanus cajan and its characterization through UV-Vis Spectrophotometry, Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), DLS Size Distribution and Zeta potential and biofilm accumulation analysis by Epi Florescence Microscopy. Antifouling is the specially designed materials and coatings to remove or prevent biofouling. The antifouling effect of the biosynthesized AuNPs was tested against marine biofilm forming bacteria on SS304 metal coupons and the results suggested that it could effectively inhibit biofilm formation. This preliminary study has proved that Cajanus cajan mediated AuNPs may be used as antifouling agent for the prevention of biofouling in the early stages.