Assessment of the Bacterial Biodiversity of Halophilic Bacteria at Akola District

The interest of current research work lies in the fact that, soil is the richest source of microorganisms and many efficient enzyme producing microorganism can be produced by soil. Akola District is reported to have saline belt spread over an covering large region of Purna river tract. Present investigation has been undertaken to study the isolation of halophilic organism from saline belt of Akola District. Total 30 samples from different saline sites were collected from various 6 areas. Total 10 isolate were obtained, out of which 5 isolates were halophilic where as 5 were non halophilic. Identification was done on the basis of Bergeys manual. Different parameters such as temperature and pH, as well as halocline production ability of isolate by Disc Diffusion Method was also carried out. The results was recorded in terms of zone of inhibition. Isolates S2 and S4 shows very less zone of halocin production i. e 0.8 mm and 0.9 mm. Very excellent and promising result was obtained in case of S5 which shows 2.6 mm zone indicating better halocin ability. Biosurfactant activity of the obtained isolates was studied and surprisingly best result was recorded.