The Effect of BAP and NAA Treatment on Micropropagation of Cucumis sativus.L

Japanese cucumber is one of the cucumber varieties that become popular among people through out the world as salad. Recently it become popular among Malaysian. There is an increasing activities in growing this plant among farmers. One of the obstacle arises in growing this plant by farmers in Malaysia is the lack of planting materials. Seeds have to be imported from Takii company in Japan. If these F1 seeds are germinated, grow, flowered, and pollinated among each other, the offsprings at the second filial generation will not be identical to its parent plant. Micropropagation can be an alternative approach to produce offsprings that is identical to its parent plant. Therefore this experiment is carried out to micropropagate Japanese cucumber F1 hybrid ( Cucumis sativus L) using shoot tip explants. These explants are cultured in MS medium containing different concentration of BAP (6-Benzylamino purine) (1, 2.5, 5, and 10mg/L) for shoot multiplication. Shoots are rooted in a medium containing NAA (Naphthalene Acetic Acid) at 0.1, 0.5, 1.0, and 2.5mg/L All the experiments are conducted using Complete Randomized Design (CRD). Data are analyzed using analysis of Variance (ANOVA) and Duncan New Multiple Range Test. It is expected that certain concentration of BAP and NAA can maximize shoot regeneration and rooting in C. sativus F1 hybrid and the protocol for micropropagation of this crop be developed.