Molecular Characterization of Selected Hybrid Fusants of the Parent Strains of L. edodes

Five hybrid fusants viz. , L4, L13, L31, L58, and L76 were selected on the basis of horizontal radial growth after protoplast fusion carried out between LeS and LeC strains of Lentinus edodes. A total of 4 malate dehydrogenase isozymes loci were observed all together. Hybrid strains (L4 and L58) showed two bands at the Rm values of 0.33 and 0.50. Hybrid L31 strain exhibited two bands at the Rm value of 0.43 and 0.50, while L76 showed two bands at the Rm value of 0.33 and 0.40. Hybrid L13 produced no band on gel. It was observed that hybrid L4, hybrid L31, and hybrid L58 shared Rm value with LeS while hybrid L13 and hybrid L76 strains shared Rm value with LeC in case of peroxidase. Only one isoform of isozyme was observed for superoxide dismutase which was common in all the strains including one parental strain viz, LeC and 5 hybrid fusants viz, L4, L13, L31, L58, L76 except LeS. The combined effect of all the primers on all the genotypes indicated that there was no 100 % similarity between any 2 genotypes. Maximum similarity coefficient of value 0.950 was obtained between hybrid fusants L58 and L13. A combined dendrogram was obtained from the RAPD data of all the four primers together and it was found that three hybrid fusants i. e. L58, L13 and L31 were closer to LeS parent strain.