Production of Gibberellic Acid by Bacillus siamensis BE 76 Isolated from Banana Plant (musa spp)

Based on morphological, cultural, biochemical and 16s rRNA gene sequencing a newly isolated endophytic bacteria from the banana plant (musa spp) was identified as Bacillus siamensis and designated as BE 76. Fifteen bacterial isolates were screened out for their productivity of Gibberellic acid by spectrophotometeric method. Out of these isolates BE-76 isolate showed high amount of Gibberellic acid production supplemented with and without L tryptophan in medium (0.180 and 0.240 mg/mL, respectively). Gibberellic acid production of BE-76 was further confirmed by high performance liquid chromatography (198.16 and 254.29 ppm, respectively) with and without L-tryptophan in medium for more accuracy.