Isolation of m-RNA from the Blood Samples of Neonatal Jaundice Patients and then Studied the Expression of Glucose 6-Phosphate Dehydrogenase (G6PD) Gene through cDNA Synthesis and Amplification

Diseases can be caused either due to genetic factors or physiological factors. But, there are some diseases which can occur due to both of these affects e. g. neonatal jaundice. Main characteristics of the disease are hyperbilirubinemia and yellowing of the skin of patient. Genes play important role in causing the disease which control the enzymes that are responsible in bilirubin excretion process, and epigenetic factors are also responsible which regulate transcription of these genes. One enzyme that we studied was glucose 6-phosphate dehydrogenase which is responsible in causing neonatal jaundice. So, to study the role of epigenetic factors in glucose 6-phosphate dehydrogenase deregulation in neonatal jaundice patients present work was performed