Genetic Diversity of Himalayan Region Chicken through SSR Molecular Markers

Samples of chicken blood are collected from the lower Himalayan region (Red fowl and Black fowl) to study the genetic diversity on the basis of microsatellite ADL0306 whose phenotypic properties are very helpful to chicken breeders. Simple Sequence Repeats PCR technique is a reliable technique to study the difference and similarity among different species of gallus gallus which is based on repeating motifs. SSR-PCR gel electrophoresis image is analysed using Totallab Quant version 12.4 software. Software analyses gel image with respect to ladder run along with samples and generates reports in term of dendrogram and molecular weight of each electrophoresis band. Dendrogram shows close relatedness and diversity distance among all samples.