Novel Determinations of Nano-extracted Destruxin from Metarhizium anisopliae against Ephestia cautella and Ephestia Kuehniella (Lepidoptera-Pyralidae) under Laboratory and Store Conditions

The Destruxin is a cyclic hexadepsipeptides produced by entomopathogenic and phytopathogenic fungi five amino acids and one hydroxyl acid. The effect of Destruxin and nano-Destruxin on the red flour beetle -Tribolium castaneum and confused flour beetle - Ephestia Kuehniella (Lepidoptera-Pyralidae), under laboratory and store conditions were studied. The half life period LC50 of Ephestia cautella when treated with different concentrations of the fungi toxin show recorded.111X104 and 46X104 spores / ml for destruxin and nano- destruxin treatments. The half life period LC50 of E. Kuehniella recorded that, 121X104 and 32 X104 spores/ ml after destruxin and nano-destruxin treatments. Also, results, show the effect of both toxin examined against E. cautella biology, detected that, the number of eggs laid /female obtained were significantly decreased to 501.7 and 283.9 eggs/female after destruxin and nano-destruxin treatmens as compared to 3698.1 eggs/ female in the control.