Role of Alcoholic Extracts of Eruca sativa or Petroselinum crispum on Dioxin-Induced Testicular Oxidative Stress and Apoptosis

The present study was undertaken to examine the antioxidant potential of alcoholic extract of Petroselinum crispum (Pc) or Eruca sativa (Es) against TCDD testicular toxicity in male albino rats. Forty two adult male rats were allocated to one of seven groups, six rats each Control (G1), Corn oil (G2), PC (G3), Es (G4), TCDD (G5), Pc + TCDD (G6) and Es + TCDD (G7). All treatments were made by gastric intubation for 5 weeks. TCDD treated rats showed significant increase in testes lipid peroxidation products (LPO), and protein carbonyl content (PC) as well as nitric oxide (NO) associated with significant decreased superoxide dismutase (SOD) & catalase (CAT) activities, reduced glutathione (GSH) level and increased apoptosis as indicated by transforming growth factor 1 (TGF 1) and CD95. Whereas, alcoholic extract of either Pc or Es have an effective antioxidant property as observed by the normalization of antioxidant enzymes and decreased levels of LPO, PC and NO in testes co-treated with Pc or Es. Also, alcoholic extract of Pc or Es have significantly decreased apoptosis induced by TCDD. It can be concluded that alcoholic extract of Pc or Es suppress the toxic effects of TCDD by its free radical-scavenging and potent antioxidant activity.