Antibiotic Resistant Pathogens in Human Environment (ATM Machine)

Nowadays antibiotic resistant pathogens become a major problem in medical history. In this present study multidrug resistant pathogens like Bacillus cereus (bacteria) and Aspergillus sp. (fungi) was isolated and identified from human environment. Swabs were taken from different area public usage environment like bus stand and ATM machine. Swabs was further inoculated in various nutrient medium for isolation. After isolation of bacterial strain it was identified using biochemical analysis, and then it was tested against 10 type of antibiotics like Methicillin, Tetracycline, Ampicillin, Ciproflaxine, Neomycin, Oflaxin, Ceftizoxime, Cefuroxime, Cefdinir, Amoxylin, for bacterial analysis. Antibiotic sensitivity was made using Kirbey Baeyer method. resistant strain was observed and DNA sequence analysis was taken. following the same resistant fungi was also isolated and tested against antibiotics like Flucnazole, Amphotericin, Doxycycline, Metrinozole, Cycloheximide.