Measurement of Total Alkaline Phosphatase Activity in Human Serum using Charcoal Activated Para-Nitro Phenyl Phosphate

A one step Charcoal treated Alkaline phosphatase (ALP) reagent has been developed without affecting its basic ingredients. Conventionally, ALP measurement is based on the detection of phenol produced by an enzymatic reaction in 37 OC at pH10 using substrate para-nitrophenyl phosphate (pNPP). In present study, measurement range of ALP activity was noted from 6.41 to 628 U/L in serum samples. Experimental results are compared to these obtained by spectrophotometric measurement in bovine serum. Excellent Correlation exists and close agreements are noted between the both charcoal treated and without charcoal treated reagents having similar substrate (pNPP) properties. Use of charcoal has great advantages in delaying photo degradation of pNPP improving its shelf life & stability.