Mammalian Expression System and Improvisation for High Production

Recombinant proteins are of immense value both for research as well as for therapeutic use. Ever since insulin was introduced for therapeutic use there is lot of interest in the development of recombinant proteins as therapies. Recombinant proteins are often produced in heterologous host such as Bacteria, Yeast, Mammalian Cells, Transgenic Plants and Animals. Each one of these host cell lines have their own advantages and disadvantages. With the increase in the number of monoclonal antibody (mAb) based therapeutics at different stages of clinical trials is demanding for an efficient mammalian expression system. Mammalian expression system is often known to produce proteins with proper post-translational modification that were equivalent to that of humans. This review article is focused on strategies for increasing proteins production, host cell lines and the applications and products that were produced so far using mammalian expression systems. The purpose of writing this article is to summarise the advances in mammalian expression systems and their use.