Inhibition and Anti Proliferation Human Tongue Cancer Cells Supri's Clone-1 Invasion with Cyclooxygenase-2 Inhibitor

The aim of this study was to examine inhibition and proliferation of tongue cancer cells by using the SP - C1 chemopreventive drug, Celecoxib. In the method of invasion, tongue cancer cells treated with SP - C1 Celecoxib at concentrations of 5 m, 10 m, 25 m, 50 m, 75 m, 100 m and 125 m, and 0 as the control group. Tests were carried out for 24 and 48 hours with the calculation of the mean tongue cancer cell invasion SP-C1. Invasion tests used Boyden Chamber Assay and polycarbonate membrane. In proliferation test, Tongue cancer cells SP-C1 were treated with various concentrations of Celecoxib and time.192 cancer cells was grouped in 4 different groups of observation time, each group have 8 cancer cells, one without Celecoxib treatment (control), 5 m, 10 m, 25 m, 50 m, 75 m, 100 m and 125 m. Mean growth inhibition of cancer cells SP-C1 was calculated using Biorad Microplate Reader 540 nm. The results of the study showed the average number of tongue cancer cell invasion SP-C1 after the administration Celecoxib based on the concentration and time with ANOVA gives F = 60.46, which statistically significant. In zero concentration of Celecoxib (control) was 24.4 with Celecoxib concentrations ranging from 5 to 125 had decreased from an average of 11 to 2.3. In a proliferation test, results were statistically significant. Time factor (calculated F = 45.78), concentration factor (F count = 17.77), and time and concentration interaction (F count = 46.64) had showed the effect of different views of the average cell growth inhibition. The conclusion of this research Celecoxib delivery on tongue cancer cells showed SP - C1 barriers invasion and proliferation. Greater concentration of Celecoxib administered will provide a greater effect on the barrier invasion and proliferation of tongue cancer cells SP-C1.