Comparison of Three Methods for the Detection of Biofilm Formation by Clinical Isolates of Staphylococcus aureus Isolated in Casablanca

Biofilms are group of microorganisms encased in a matrix of extracellular polysaccharide (slime), called polysaccharide intercellular adhesin (PIA). They have been associated with a variety of chronic and persistent infections. Staphylococcus aureus is from bacteria which have high ability to form a biofilm. In this study, detection of biofilm production by Staphylococcus aureus was done by using three different methods tube method (TM), congo red agar method (CRA) and tissue culture plate method (TCP), three repetitions were made for every method. Among of 117 strains of Staphylococcus spp isolated between October 2015 and January 2016 at the University Hospital Ibn Rochd in Casablanca, 74 were identified as Staphylococcus aureus.58 isolates were detected as biofilm producer by TCP method, 49 by TM and 42 by CRA method. We can conclude from our study that the TCP method is a more quantitative and reliable method for the detection of biofilm formation by Staphylococcus aureus as compared to TM and CRA methods.