Isolation, Identification and Antibiotic Sensitivity Pattern of Bacteria from Urine Samples in Erbil Hospitals

Seventy one samples of urine were collected from patients suffering from signs and symptoms of urinary tract infections (UTIs) admitted to Erbil hospitals (Erbil teaching hospital, Maternity teaching hospital, Rizgary teaching hospital and Raparen hospital) and Medya diagnostic center during the period from July 2011 to August 2011. The urine samples were cultured on Blood and MacConkey agar, to detect and identify causative bacteria. The results indicate that the positive samples were 50 (70.40 %), while negative samples were 21 (29.60 %), out of this 32 (64 %) represent female while 18 (36 %) represent male. The isolated bacteria were identified according to cultural characteristics, microscopical examination and biochemical reaction in addition to Api 20 E system. Escherichia coli was among the most predominate pathogenic bacteria isolated from the urine with a rate of (62 %) while other bacteria were Klebsiella pneumoniae (10 %), Proteus mirabilis (8 %), Pseudomonas aerugenosa (8 %), Staphylococcus aureus (6 %), Enterobacter spp. (4 %), and Staphylococcus saprophyticus (2 %). Sensitivity test of bacterial isolates to different antimicrobials (Amikacin, Ampicillin, Cefteriaxone, Ciprofloxacin, Nalidixic, Gentamycin and Imipenem) were done. The isolated bacteria showed sensitivity to Imipenem and low resistance to Amikacin, variation in resistance to Ceftriaxone, Ciprofloxacin, Nalidixic and Gentamycin, however, they showed high resistance to Ampicillin.