Analytical Method Determination of Betulinic Acid: A Review

Betulinic acid is the pentacyclic triterpenoids which occurred naturally then exhibits the potent anticancer activity. This review aims to compiling the published analytical method for the determination of the Betulinic acid. By using the techniques like UV visible spectrophotometry, High Performance Liquid Chromatography(HPLC),High Performance Thin Layer Chromatography (HPTLC) Tandem Mass Spectrometry, LC/MS/MS, Ultra Performance Liquid Chromatography (UPLC),Thin Layer Chromatography (TLC). Further method validation is carried out to ensure the method developed was accurate, specific, reproducible, precise over the specified range in which an analyte is analysed.