Synthesis and Characterization of Benzimidazole Derivatives for Antimicrobial Property

Benzimidazoles are an important class of compounds with a wide spectrum of biological activity ranging from anti-hypertensive, anti-viral, anti-microbial, antitumor and anthelmintic activity. Benzimidazole rings are the most important nitrogen-containing heterocycles, which are widely explored and utilized by the pharmaceutical industry for drug discovery. Due to their special structural features and electron-rich environment, Benzimidazole containing drugs bind to a variety of therapeutic targets, thereby exhibiting a broad spectrum of bioactivities. Numerous benzimidazole based drugs have been extensively used in the clinic to treat various types of diseases with high therapeutic potential. The main objective of present work was to study the anti-microbial activity of the Benzimidazole derivatives. A series of benzimidzole derivatives have been synthesized and identified. The compounds were synthesized by using ethyl acetate and benzene as starting material. The series of 1, 2-disubstituted benzimidazoles containing pyrimidine and other functional groups was prepared which provides advantages such as, easy workup and high yield. All reagents used for synthesis were of synthetic grade. Purification of all compounds was done by thin layer chromatography using silica gel G as absorbent on glass plate using acetate: benzene (6:4 v/v %), Toluene: Acetone (8:2 v/v %) and Ethyl Acetate: n-Hexane (6:4 v/v %) as mobile phase. Compounds were detected by using iodine vapor as detecting agent. All compounds show single spot. All the newly synthesized compounds were characterized by IR spectral study. The compounds were investigated for their antimicrobial activity against clinical standard drug Ciprofloxacin. The anti-microbial study of the synthesized derivative was done Broad panels of bacterial and fungal strains were used for testing the antimicrobial properties of the synthesized molecules III1-13. The compounds III 1 (m-NO 2), III 2 (p-NO 2), III 3 (m-Cl), III 4 (3-F-4-Cl) and III 9 (p-OCH 3) showed excellent activity (62.5 μg/ml), even better than ciprofloxacin.