INFLUENCE OF 627 NM WAVELENGTH LIGHT EMITTING DIODEPHOTOTHERAPY ON SECONDARY INTENTION WOUND HEALING

Background: The use of photobiomodulation can be effective in the healing of tissues, highlighting the use of LED in tissue repair. Was evaluated the effect of 627 nm wavelength LED irradiation on VEGF-α and TGF-β1 expressions in a model of secondary intention wound healing. Methods: The evaluations were performed using a dorsal puncture model and the sample consisted of 120 Wistar rats, randomly divided into LED groups submitted to 3, 7, 14 and 21 irradiation treatment for 100 s, 7 J / cm2, and control groups. Histological analysis, immunohistochemistry and contraction of the lesion were performed. Results: LED irradiation led to effective wound regeneration results. Similar effects were obtained with vascular, exudative, and proliferative phenomena. VEGF and TGF-β1 levels were increased in LED-treated groups with initial treatments. Collagen analysis revealed that LED-treated groups levels were increased on days 14- and 21 of the treatments. An acceleration in tissue regeneration was observed in LED treated groups. Macroscopically visible results showing a complete healing process was evident after 14 days of treatment. Conclusion: 627 nm LED irradiation effectively accelerated wound healing leading complete closure of the skin in a shorter time compared to control group.