A STUDY ON ANTIBACTERIAL ACTIVITY AND EXTENSION OF SHELF LIFE OF FRESH COW URINE

Cow urine has been used as medicine since ancient times andthe ancient scriptures of Ayurveda consider cow urine to be the elixir of life. The present study analyzes the inhibitory effects of fresh cow urine at different pH (8.2,4.4,3.8) at the intervals of 5,10, 20,30 days and antibacterial activity of cow urine at different pH was analyzed against the Escherichia coli , Staphylococcus aureus and Klebsiella sp. It was found that no bacterial growth was present in fresh cow urine at pH 3.8 after 30 days. Shelf life of cow urine could be extended after lowering the pHwhich also makes it palatable as it becomes odourless. Maximum antibacterial activity was also observed in fresh cow urine sample at pH 3.8 against gram positive and gram-negative bacteria Escherichia coli (24mm ± 2.0), Staphylococcus aureus (22mm ± 2.0) and Klebsiella sp (22mm ± 1.0).Fresh cow urine at pH 3.8 was found to be more active and exhibited better antibacterial activity.It can be used in the control of bacteria.