The Use of Ethanol Extract of Gorek Seeds to Lower the Level of Lipid Peroxidation in Male White Mice of Wistar Strain Exposed to Cigarette Smoke

— Cigarette smoke causes an imbalance between free radicals and antioxidants, causing oxidative stress followed by an increase in lipid peroxidation. Gorek seeds (Caesalpinia bonducella) contain high phenolic, antioxidant capacity, flavonoids, and tannins that can capture free radicals. This experimental study aims to prove that the ethanol extract of Gorek seeds can reduce lipid peroxidation levels in mice exposed to cigarette smoke. The decrease in lipid peroxidation was indicated by a decrease in isoprostane F2 in mice urine. The research used a pre-test post-test control group design, with 2 groups of mice as the control group and the treatment group, exposed to cigarette smoke for 14 days. Besides being exposed to cigarette smoke, the treatment group was also given ethanol extract of Gorek seeds at a dose of 250 mg/kgBB. The results showed that the F2 isoprostane level of the treatment group decreased significantly from an average of 4.68±0.71 ng/mL to 3.52±0.44 ng/mL (p<0.05 ), while the F2 isoprostane level of the control group increased significantly from a mean of 4.75±0.42 ng/mL to 5.04±0.34 ng/mL (p<0.05). These results indicate that the ethanol extract of Gorek seeds can significantly reduce lipid peroxidation level