Purification and Properties of ortho Ring Cleavage Enzyme Catechol 1, 2 - dioxygenase from Genetic Modified Strain of Pseudomonas aeruginosa

Pseudomonas aeruginosa utilize 4 - chlorobezoic acid as sole source of carbon and energy, degrading it through ortho ring cleavage pathway. They synthesized catechol 1, 2 - dioxygense enzyme which is unique its properties to transform catechol to cis, cis - muconic acid. The procedure for purification and homogeneous nature was describe by polyacrylamide gel electrophoresis. The molecular weight of enzyme was 55 kDa estimated by agrose gel electrophoresis. The subunit size was 25 kDa determined by SDS - PAGE.