Chemical Identification of Volatile Oil of Nigella Sativa and Evaluation of its In-Vivo Wound Healing Activity

To screen the wound healing activity of oil of Nigella sativa in excision, incision and burn wound models in albino rats. The seeds of Nigella sativa. (Family Ranunculaceae) were grinded by a suitable grinder and was subjected to continuous extraction with the steam on steam distillatory. Volatile oil has been separated out and collected in a tightly closed container. The extract was tested for various preliminary phytoconstituents, the chemical structure has been identified by Infrared spectroscopy (IR) method and screened for wound healing activity in excision, incision and burn wound models in albino rats at the dose of 5% (v/v), 10% (v/v) in LLP formulation by topical administration. Oil of seeds of Nigella sativa has shown better wound healing activity in excision, incision and burn wound models as compared to povidone iodine (used as standard drug in excision and incision wound model) and silver sulfadiazine (used as standard drug in burn wound model). From the results obtained it can be concluded that extract of seeds of Nigella sativa has significant wound healing activity.