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HPLC Fingerprint Analysis with in - vitro Antimalarial Activity and Bioassay Guided Fractionation of Two Organic Extract from Ageratum conyzoides Leaves

Journal: International Journal of Science and Research (IJSR) (Vol.10, No. 10)

Publication Date:

Authors : ; ;

Page : 1471-1476

Keywords : Ageratum conyzoides; antimalarial activity; fractionation; HPLC; Thin Layer Chromatography; TLC;

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We investigated the antimalarial activities of fractions from cyclohexane and dichloromethane extracts of Ageratum conyzoides Leaves. These extracts were fractionated using preparative - TLC and six fractions were obtained for each extract. The antimalarial activity of these fractions was evaluated against Plasmodium falciparum. Fractions 4 (F4) and 5 (F5) from dichloromethane extract showed the best antimalarial activities against the parasite with IC50 values of 0.25 and 0.30?g/mL, respectively. The HPLC profile of these most active fractions were developed and showed two and three major peaks respectively for fraction 4 (F4) and 5 (F5). Preliminary phytochemical screening revealed the presence of alkaloids, steroids and terpenoids in the fraction 4, whereas only steroids and terpenoids were found in the fraction 5 (F5). Thus, these fractions could be considered as the prospective fractions to be developed as antimalarial drugs.

Last modified: 2022-02-15 18:46:47