Genetic Polymorphisms at Myostatin Locus in Fat Tailed Sheep, Dorper and Texel Sheep

The objectives of the research was to analyze genetic diversity and genetic polymorphisms at Myostatin gene locus in Fat Tailed Sheep, Dorperand Texel Sheep. Research was conducted in the village area of Pandesari, Pujon District, Malang Regency, East Java Indonesia. Research was carried out in two stages, (1) field research, to observe the linear measurements of Fat Tailed Sheep, Dorper Sheep and Texel Sheep. Blood samples were taken from 91 sheep consisting of the three breeds35 (Texel, 19 Dorper and 37 Fat Tailed Sheep). (2) Laboratory research was conducted to analyze DNA polymorphism with target gene was the myostatin gene on exon 3, using PCR-RFLP analysis with Msp1 restriction enzyme was conducted to analyze polymorphism at the myostatin gene in these sheeps. The Msp1 was used to cut the mutated fragments. Data analysis used (1). Analysis of % PIC to analyze degree of polymorphism, (2) For linear measurements data such as chest girth, body length, body height, tail circumference was analysed using Minitab software version 17. Results showed that from the aspect of the phenotype, Texel, Dorper and DEG sheep have a high level of diversity, which is quite high (above 9%). The results of PCR-RFLP obtained monomorphicsite in all breeds of sheep so that the correlation between MSTN gene polymorphisms and sheep growth could not be analyzed. Because the Myostatin gene of the three breeds was monomorphic. It was concluded that the exon 3 area in all breeds was a conserved region that rarely occurs mutations.