Peptide Based Vaccine against Human Herpes Virus-7 Using in Silico Approach

Background: Human Herpesvirus 7 (HHV-7) appears to be principally acquired in infancy and childhood and able to cause roseola. No such vaccine is available that can cure the virus with cross-protection. Thus this study involves a peptide based vaccine design with the help of immunoinformatics approach. Objectives: To design a peptide based vaccine for herpes virus-7 using immune informatics tools. Materials and Methods: sequences of envelope glycoprotein H were retrieved from National Center of Biotechnology Information (NCBI) database in 2020 and aligned to determine the conservancy between the retrieved strains. The Immune Epitope Data Base (IEDB) different analysis resources were used to predict epitopes that could act as a peptide vaccine against herpes virus 7. The predicted epitopes were further assessed for population coverage against the whole world population. Results: The epitopes 81-FDQYKHR-87, 141-IRKLYYNQ-148, 478-KDLTQRVV-485, 342-LLYPEMEKL-350, 572-CTPTNYKYS-580, 97-EKAVKIYAQ-105, 106-KFQTNIKPV-114 and 506-SVYRKKRLDM-515 were found to be the most potential eight epitopes against B cells. For the T cell Three epitopes namely, 180-FMLALTPSF-188 , 238-TTIERFYPF-246 and 616-YIMDDKQLL-624 showed high affinity to MHC-I alleles , The Three epitopes (core) 347-MEKLQNFQL-355 , 50-YNETRVYQI-58 and 512-RLDMFKSIS-520 showed high affinity to interact with MHC-II alleles, high coverage for whole world population with percentage of 80.70% and 61.02% respectively. Conclusions: This study proposed eight epitopes for B and six for T cells that could be a powerful multi epitope vaccine, Clinical trial like experimental animals is required to proof the efficacy of these epitopes as promising candidate vaccine against human herpes 7.