Isolation and In Vitro Activities of Asplenum Bulbiferum Leaf Extracts on Bacteria Associated with Lung Diseases

Lung diseases have been a worldwide health problem, with high endemicity in developing countries. Bronchopneumonia remains the main disease of the lung. Despite advances in modern medicines, there is no effective drug available that stimulate lung function, offer protection to the lung from damage or help to regenerate pulmonary cells destroyed by disseminated bacteria. There is an urgent need for effective drugs to replace supplement those in current use. This study was embacked upon to evaluate the in vitro effect of Asplenium bulbiferum leaf extracts on isolates from patient's sputum. Streptococcus pneumoniae was obtained from human specimen, identified through cultural, morphological and biochemical examination. The antibacterial activity was carried out using agar well diffusion technique. Tube dilution technique was used to determine the minimum Inhibition Concentration MIC and Minimum Bactericidal Concentration MBC using double fold serial dilution at concentrations of 400mg ml to 25mg ml. The photochemical analysis revealed the presence of alkaloids phenolics, tannins, saponins, flavonoids, steroids and glycosides. The ethanolic extract exhibited more activity than the aqueous extract against S. aureus most followed by S. pneumoniae, H. influenza and K. pneumoniae was the least. The study showed that Asplenium bulbiferum possessed antibacterial properties and should serve as alternative therapy for ameliorating lung infections. Eze, H. C | Ajogwu, T. M | Ozor C. N | Gloria, C. I | Soludo, O. C "Isolation and In-Vitro Activities of Asplenum Bulbiferum Leaf Extracts on Bacteria Associated with Lung Diseases" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd47621.pdf Paper URL : https://www.ijtsrd.com/biological-science/microbiology/47621/isolation-and-invitro-activities-of-asplenum-bulbiferum-leaf-extracts-on-bacteria-associated-with-lung-diseases/eze-h-c