A NEW BOON IN CHROMATOGRAPHY UPLC ?A REVIEW Swapnil Anil Palve*, Swati Gokul Talele, Dr. Ghanashyam Chaudhri

Abstract: Miniaturization embracing instrumentation, column particle size and column dimensions is one of major current trends in separation techniques. High performance liquid chromatography (HPLC) has utilized most widely in analytical laboratories. Though HPLC is dynamic and versatile technique, there is critical necessity for reliable, faster, more accurate chromatography technology. UPLC technique in chemistry and instrumentation accepted widely due to its ability to provide more information per unit of work as UPLC fulfills the criteria like increased speed, resolution and sensitivity predicted for liquid chromatography UPLC is preferable for obtaining chromatographic separation where packing material have particle size less than 2.5μm. Today’s pharmaceutical companies give more emphasis on to cut cost and lessen time for development of new drug with improvement in quality of the products. A typical assay was transferred and optimized for UPLC system to achieve both higher sample analysis throughput and better assay sensitivity. Analysis of operation cost and sample throughput found UPLC cost advantageous over HPLC. UPLC is a rising chromatographic separation technique whose packing materials have smaller particle size lesser than 2.5μm which improves the speed, resolution and sensitivity of analysis. When many scientists experienced separation barriers with conventional HPLC, UPLC extended and expanded the utility of chromatography. The main advantage is a reduction of analysis time which also reduces solvent consumption. The analysis time, solvent consumption and analysis cost are very important factor in many analytical laboratories. The time spent for optimizing new methods can also be greatly reduced. Key words: UPLC, chromatography, HPLC, Instrumentation.