RESTORATION OF THE STRUCTURAL AND FUNCTIONAL STATE OF ERYTHROCYTES AFTER HYPOTHERMIC STORAGE USING HUMAN CORD BLOOD LOW-MOLECULAR FRACTION AND THE DRUG ACTOVEGINJournal: Biotechnologia Acta (Vol.14, No. 6)
Publication Date: 2021-30-12
Authors : O. K. Gulevskyy Yu. S. Akhatova;
Page : 44-52
Keywords : cord blood; Actovegin; erythrocytes; hypothermia; recovery medium.;
One of the modern transfusiology tasks is to preserve the properties of erythrocytes after hypothermic storage. The transfusion medium quality and efficiency depend on their functional state. Plausible protective effects of a human cord blood low-molecular fraction (CBF) and the drug Actovegin were evaluated in the study in order to use them as components of recovery medium. Aim. The purpose of this study was to investigate the CBF and the drug Actovegin effect on erythrocytes morphology, energy balance, and oxygen transport function of erythrocytes after their hypothermic storage. Materials and methods. Erythrocyte mass was stored in hypothermia for 7–21 days. Every 7 days, the CBF or Actovegin (final concentration 0.6 mg/ml) were added to the samples and incubated for 1 h at 37 C. CBF was obtained from human cord blood by ultrafiltration. The erythrocytes morphology was assessed using light microscopy. The content of ATP and 2.3-DPG was determined by photoelectrocalorimetric method. The saturation, О2 and СО2 tension were assessed using an analyzer of blood gases. The ratio of hemoglobin forms was studied by photometry. Results. It has been shown that both CBF and the drug Actovegin helped to restore the morpho- functional characteristics of erythrocytes after 21 days of their storage at 2–4 C. This was indicative as an increase in the normocytes number increase and restoration of О2 tension, saturation, ATP and 2.3-DFG content, and normalization of the hemoglobin forms ratio. It is suggested that the mechanisms of the drags action was are associated with ATP synthesis activation and 2.3-DFG formation. Conclusions. The use of media containing CBF or the drug Actovegin enabled to effectively restore the properties of erythrocytes disturbed after their prolonged storage.
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