Study of Phytochemical and Antioxidant Activity of Hydro-methanolic Extract of Trigonella foenum-graecum Seed and Indigenous Cow Urine Distillate in Broiler Birds

Oxidative stress in broiler chicken can result in damage to biomolecules, cells and tissues which thereby can decrease immunity, antioxidant system and result in poor growth rate and production of the bird?s. Present study was conducted to evaluate the effects of supplementation of hydro-methanolic extract of fenugreek seed extract (1g/L in drinking water) on oxidative stress in broiler birds. In this experiment phytochemical analysis and antioxidant parameters were studied. Dried seed powders of fenugreek seed were extracted with hot methanol by using Soxhlet?s apparatus. Hot hydro-methanolic extract of Fenugreek seed was referred hereafter ?Fenugreek Seed Extract? (FSE). For study of antioxidant status one hundred fifty-day old Ross AP broiler birds were used and randomly divided into five groups namely control, standard, FSE (T1), CUD (T2) and FSE+CUD (T3). Each group consisted of 3 replicates with 10 birds. The control group was supplemented with basal diet. standard group with 0.25% Enradine and groups FSE, CUD and FSE+CUD were supplemented 1g/L of fenugreek seed extract, Cow Urine Distillate (10 ml/lit) and its combination respectively in drinking water daily for consecutive 42 days. Phytochemical analysis was assessed by different chemical method and results revealed the presence of Phenolic compound, flavonoids, saponin, terpenoid, glycosides etc. Antioxidant status were assessed by MDA level in lipid peroxidation, Reduced Glutathione and Glutathione peroxidase. Increased level of glutathione peroxidase, glutathione and reduced level of lipid peroxidation were observed in this study. Thus, confirming the antioxidant / scavenging action of FSE and this might be due to the presence of many potent phytochemicals flavonoids, polyphenols, terpenoids etc. Therefore, it can be used for ethno veterinary medicine purposes.