PROTECTIVE ROLE OF α-MERCAPTO- β-ACRYLIC ACID SUBSTITUTED DERIVATIVES ON OXIDATIVE STRESS, LIPID PEROXIDATION, ANTIOXIDANT ENZYMATIC ACTIVITIES IN CADMIUM TOXICITY INDUCED RAT MODEL

Objective: Synthesize some new α-Mercapto-β-acrylic acidsubstituted derivatives, characterize them and investigate their protective role on oxidative stress, lipid peroxidation, antioxidant enzymatic activities and Cd levels in the blood and tissues of cadmium exposed rats. Method: Twenty-four male rats were divided into three groups of eight rats each. The control group received distilled water whereas group II received CdCl2 (1.5 mg/4 ml/body weight) through gastric gavage for 21 days. Group III to Group VI received CdCl2 and was treated with synthesized compounds for 21 days. The lipid peroxidation level, oxidative stress and enzymatic parameters were measured in plasma and tissues (brain, liver and kidney) of all groupsα-Mercapto-β-acrylic acidderivatives were significant in reinstate normal blood profile and biochemical enzymatic levels of Cd-induced toxic animals. Result: C4 derivative was most effective by restoring hemoglobin level to 11.05 g/dL and reduced Cd level in blood to 4.64 µg/mL as compared to toxic control group. Xanthine oxidase (XO) and Glutathione-S transferase (GST) activities were significantly altered in the plasma and tissues of the CdCl2 induced group as compared with the control group. Total thiol level was significantly (p<0.001) decreased in α-Mercapto-β-acrylic acidderivatives treated group as compared to cadmium exposed group. Reduction in the MDA level and MPO level was more significant with C4 synthesized compound among other synthesized compounds. Conclusion: Treatment with α-Mercapto-β-acrylic acidderivatives decreased the cadmium level in blood and tissues in all groups. without any major side effect, α-Mercapto-β-acrylic acidderivatives were found effective in reducing Cd toxicity in rats. We can conclude that the synthesized α-Mercapto-β-acrylic acidderivatives are effective against Cadmium toxicity.