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Antimicrobial Activities of Irvingia gabonensis Leaf and Cyperus esculentus Extracts on Some Selected Isolates | Biomedgrid

Journal: American Journal of Biomedical Science & Research (Vol.7, No. 3)

Publication Date:

Authors : ; ;

Page : 233-236

Keywords : Bush mango; Tiger nut; antibacterial activity; Zone of inhibition; Ethanolic extract;

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Bacterial resistance to antibacterial drugs in the treatment of some bacterial infections have become a menace that is causing untold health challenges to patients. The antibacterial activity of ethanolic extract of tiger nut (Cyperus Esculentus) and Bush mango (Irvingia Gabonensis) against candida albicans, Echerichia coli, Pseudomonas sp, Salmonella sp and Staphylococcus aureus was evaluated using agar-well diffusion, Minimum Inhibitory Concentration (M.I.C) and Minimum Bacteriocidal Concentrations (M.B.C) methods. Echerichia coli had the highest total colony count of 1.3 X 1011 Colony forming unit/ml(Cfu/ml) followed by Pseudomonas sp (8.8 X 1010Cfu/ml) and Staphylococcus aureus (6.7 X 1010) at 1.0E-09 dilution, but in the 1.0E-10 dilution, Pseudomonas sp was highest with 9.7 X 1011Cfu/ml followed by E. coli (9.0 X 1011Cfu/ml).The result from the comparative agar well diffusion assay of Tiger nut and Irvingia crude extracts showed that the extracts have antimicrobial activity against some of the test isolates. Pseudomonas spp. were susceptible to the tiger nut extract (10% and 20%) with the diameter of Zone of Inhibition (ZOI) of 18.0±0mm and 18.5±1.5mm. While Echerichia coli, Pseudomonas sp, Salmonella sp and Staphylococcus aureus showed susceptibility to the Irvingia (10%) crude extract with ZOI diameters of 13±2mm, 15.5±0.5mm, 16.5±1.5mm and 16.5±0.5mm respectively. Pseudomonas also exhibited susceptibility to the Tiger nut (20%) crude extract having a ZOI diameter of 18.5±1.5 mm while E.coli, Pseudomonas spp, Salmonella spp and Staphylococcus aureus were susceptible to Irvingia (20%) crude extract, revealing ZOI diameters of 15.5±0.5 mm ,19±0 mm,18±1 mm and 15±0 mm respectively The Minimum Inhibitory Concentration (MIC) of both extracts was 50% for all organisms, while the Minimum Bacteriocidal Concentration (MBC) of both crude extracts was 50% for Staphylococcus aureus only. The dilution factors and total colony counts from 1.0E-09 and 1.0E-10 dilutions of 5 test isolates. where statistically analyzed with ANOVA, and the P. values where 0.13 and 0.4 respectively were significant.

Last modified: 2022-06-11 16:38:23