Meclizine Chloridrate and Methyl-β-Cyclodextrin Associated with Monophosphoester Synthetic Phosphoethanolamine Modulating Proliferative Potential in Triple-Negative Breast Cancer Cells

Synthetic phosphoethanolamine (Pho-s) is a monophosphoester ester with anti-inflammatory and pro-apoptotic properties. Meclizine chloridrate (MC) is a histamine H1 receptor blocker that is also able to inhibit cellular respiration. However, MC does not inhibit cellular respiration in isolated mitochondria such as antimycin and rotenone. Methyl-β-cyclodextrin (MβCD) belongs to the β-cyclodextrin family, which is capable of removing cholesterol from the plasma membrane. The aim of this study was to evaluate the proliferative effects of meclizine chloridrate and methyl-β-cyclodextrin compounds associated with synthetic phosphoethanolamine in a triple-negative human breast tumor line, MDA-MB-231 Cell viability of the tumor line and normal cells FN1 was evaluated by MTT colorimetric test; the production of free radicals was determined by lipoperoxidation (LPO) test; and the percentage of cell cycle phases and proliferative index was evaluated by flow cytometry. Cell viability demonstrated a significant decrease with the treatments of MβCD, MC and Pho-s associated with MC. The production of free radicals decreases significantly in all treatments. In addition, a significant increase of DNA fragment and decrease in G0/G1 cell cycle phase were observed in cellular percentage with concentrations of 20 and 30 mM of Pho-s in association with MC and MβCD, respectively.